An improved method for concurrent isolation of human granulocytes and lymphocytes. Its application in isoelectric focusing electrophoresis.
نویسندگان
چکیده
A modified method of Boyum's technique to isolate granulocytes and lymphocytes from human blood is described. The granulocytes and the lymphocytes separated by this isolation procedure are highly pure, viable, and in sufficient number for subsequent isoelectric focusing studies. Different proteins have been separated by isoelectric focusing. This technique can be useful in the investigation of granulocytic and lymphocytic disorders.
منابع مشابه
Concurrent isolation of granulocytes and lymphocytes with unaltered permeability, energy state, and metabolic capacity in vitro.
Although numerous procedures have been described for the isolation of either granulocytes or lymphocytes, few focused on the concurrent separation of these cells from the same sample of blood. Those reported (e.g., (1)) are based on the use of discontinuous gradients of Ficoll-Hypaque (sodium diatrizoate), a method introduced by Boyum (2). However, a common disadvantage of these procedures is t...
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ورودعنوان ژورنال:
- Annals of clinical and laboratory science
دوره 11 2 شماره
صفحات -
تاریخ انتشار 1981